Differential expression of antioxidant system genes in. Effects of flight activity and age on oxidative damage in the. Altmetric antioxidant enzymes in the honey bee,apis. Effects of flight activity and age on oxidative damage in the honey. Overview of attention for article published in apidologie, january 2002. Evaluation of pesticideinduced oxidative stress in honey bee. Social insect, oxidative damage, reactive oxygen species, antioxidant system, apis mellifera. Honey bee apis mellifera drones survive oxidative stress due to increased tolerance instead of avoidance or repair of oxidative damage hongmei libyarlay a,b,c. Biomarkers of oxidative stress in atrazinetreated honey. Potential antioxidant and anticancer effect of apis.
Evolutionary history of the honey bee apis mellifera. Sperm storage and antioxidative enzyme expression in the. Abstract the recent decline in managed honey bee populations, apis mellifera l. Study of genetic structure of honey bee from cyprus apis. Of the 45 sites mapped with the 16 restriction enzymes assayed, 23 were found apis cerana apis melliferu 1 colony 68 colonies. Differential expression of antioxidant system genes in honey bee. Upregulation of antioxidant genes in the spermathecae of. Sperm viability and gene expression in honey bee queens. Mirroring vg levels, gene transcripts encoding antioxidant enzymes and. Mar 29, 2004 honey bee apis mellifera sperm remains viable in the spermatheca of mated female honey bees for several years. Effects of flight activity and age on oxidative damage in.
Apr 01, 2004 honey bee apis mellifera sperm remains viable in the spermatheca of mated female honey bees for several years. Evaluation of pesticideinduced oxidative stress in honey. We also evaluated the changes in the activity of antioxidant and detoxification enzymes in their body. Temporal polyethism is based on physiological differences related to the honey bee activities into the colony, suggesting a different requirement of antioxidant. Antioxidant enzymes activity during age polyethism in apis mellifera. Jun 06, 2019 in this study, we explored the effects of higher temperature and humidity on the epiphysiology of bees by evaluating the survival, tolerance and body water loss in two bee species apis cerana and apis mellifera. Oct 01, 2006 antioxidant enzymes perform a variety of vital functions including the reduction of life. Fundaments of the honey bee apis mellifera immune system. Evolutionary history of the honey bee apis mellifera inferred.
During this time, the sperm retains respiratory activity, placing it at risk of the damaging effects of reactive oxygen species common to many biological processes. Sep 01, 2018 in this study, insecticide incidence, dna damage, and antioxidant enzyme activity were studied intrareas. Previous studies report that acute exposures to the commonuse herbicide atrazine elicit oxidative stress in nontarget. In the honey bee, apis mellifera, mitochondrial activity in the larval fat body has been. In contrast, the delay in antioxidant gene expression in worker larvae may explain their endogenous hypoxia response. Nontarget inhibition of antioxidant enzymes in honey bees. One outstanding threat to honey bees is the unintended exposure to agricultural pesticides. Lytic enzymes are usually reported to have a role in fungal entomopathogenicity. Forager bees and nurse bees used for all assays were identified based on behavioral observations robinson, 1987.
Antioxidant enzymes in the honey bee, apis mellifera apidologie. A total of 38 antioxidant genes in the honey bee genome have been identified. Changes in antioxidant enzymes activity and metabolomic. Antioxidant enzymes in the honey bee, apis mellifera. Supplementation of the honey bee diet with vitamin c. Potential antioxidant and anticancer effect of apis dorsata. Antioxidant enzymes in the honey bee, apis mellifera gunter weirich, anita collins, virginia williams to cite this version. Theselected mrnas encoded catalase, glyoxalase domaincontaining protein 4like glod4l, gstd1, sod1, thioredoxin 2 txn2, thioredoxin reductase 1 txnrd1, and vitellogenin.
Staron m1, sabo r2, sobekova a3, sabova l4, legath j5, lohajova l3, javorsky p6. The effects of age on the antioxidant defense systems and prooxidant generation were analyzed by comparing the activity of catalase and rates of h 2 o 2 generation in the bees. Honey bee apis mellifera drones survive oxidative stress due to increased tolerance instead of avoidance or repair of oxidative damage hongmei libyarlaya,b,c. The purpose of this experiment is to gain an insight into the antioxidant response of honey bees and its natural microbiota towards pesticideinduced stress. Determination of some biochemical parameters of worker. Insecticide susceptibility in asian honey bees apis cerana. Pdf antioxidant enzymes in the honey bee, apis mellifera. Honey bee apis mellifera drones survive oxidative stress. Results the mean ph of apis mellifera, hypotrigona sp.
Antioxidant, antihyperglycemic, and antidiabetic activity. Insights into social insects from the genome of the honeybee. Antioxidant enzymes in the honey bee, apis mellifera archive ouverte hal. Apis mellifera has fewer genes than drosophila and anopheles for innate immunity, detoxification enzymes, cuticleforming proteins and gustatory receptors, but more genes for odorant receptors, and. Honey bees apis mellifera pollinate plants in both natural and managed. The infamous african killer bees, apis mellifera scutellata, the queens of which were introduced to brazil in 195610, are known for. Formetanate toxicity and changes in antioxidant enzyme. Enzymic profiles produced by ascosphaera apis, a pathogen causing chalkbrood disease in honey bee larvae were determined by api zym kit and standard enzyme assays. Differential expression of mitochondrial genes between queens and workers during caste determination in the honeybee apis mellifera. In this study, sandwich enzyme linked immunosorbent assay and lcms based untargeted metabolomic analysis were employed to determine the changes in the specific activities of antioxidant enzymes and the metabolomic profiles in gut tissues of a.
Jun 01, 2016 the western honey bee, apis mellifera is the most important managed pollinator of agricultural crops worldwide klein et al. Sorry, we are unable to provide the full text but you may find it at the following locations. Sister worker honeybees apis mellifera produced by a single queen bee were sampled at the beginning november 2010, approximately 6 weeks old and in the middle of winter january 2011, approximately 12 weeks old from one colony at the university apiary clujnapoca. Formetanate toxicity and changes in antioxidant enzyme system. Seasonal variation in the activity of selected antioxidant enzymes. Phenolic compounds, antioxidant capacity and bioaccessibility of.
Links hsu cy and chuang yl 2014 changes in energyregulated molecules in the trophocytes and fat cells of young and old worker honey bees apis mellifera. Evaluation of physicochemical and antioxidant properties of. The results showed that enzyme activity in bees under t, rh, th treatments was higher than the control treatments. Effects of truckmounted, ultra low volume mosquito. The substantial activities of all three enzymes in spermathecae of mated queens suggest their involvement in the longterm protection of the spermatozoa from oxidative stress. Antioxidant enzymes in the honey bee, apis mellifera gunter f.
Antioxidant enzymes in the honey bee, apis mellifera core. We used the honey bee genome sequence to identify the major components of the honey bee antioxidant system. Oxidative stress and antioxidant enzyme activities in the trophocytes and fat cells of queen honeybees apis mellifera rejuvenation res. Accepted, 304 accepted, 304 a gricultural r esearch s ervice, b eltsville a rea b ee r esearch l aboratory. Honey bee antioxidant enzymes are of particular interest because of their potential involvement in some of the exceptional biological characteristics of the queen honey bee, especially its longevity relative to worker bees 10. Honey bee colony performance and health are enhanced. For the honey bee apis mellifera the assessment of antioxidative capability became easier after the sequencing of the honey bee genome. Insecticide susceptibility in asian honey bees apis. Evaluation of physicochemical and antioxidant properties.
Jun 05, 2018 apis mellifera bee tea is used by indigenous brazilians to treat diabetes, and this traditional knowledge needs to be recorded and studied. Formetanate toxicity and changes in antioxidant enzyme system of apis mellifera larvae. Tolerance and response of two honeybee species apis cerana. Pdf gut pathology and responses to the microsporidium. Pdf the protective strategy of antioxidant enzymes. The most important antioxidative enzymes in the insect are superoxide dismutase sod. Five generations of twoway selection resulted in high and low strains that differed more than sixfold in. Honey bee rearing, collection and dissection honey bees apis mellifera linnaeus 1758 for this study were reared at the university of nevada, las vegas unlv, nv, usa apiary. Tarpya,b, olav rueppellc, a department of entomology, north carolina state university, raleigh, nc, usa b w. A comparative analysis of honey bee with drosophila melanogaster and anopheles gambiae shows that although the basic components of the antioxidant system are conserved. What is the main driver of ageing in longlived winter.
Apis dorsata binghami is sulawesi endemic honey bees, known for its giant honey bees hadisoesilo, 1997. Oxidative stress and antioxidant enzyme activities in the. Upregulation of antioxidant genes in the spermathecae of honey bee apis mellifera queens after mating alejandra n. Differential expression of antioxidant system genes in honey bee apis melliferal. Restriction sites annotated with an italic letter between parentheses are polymorphic in apis mellijm see table 1. The fungus ascosphaera apis, an obligate fungal pathogen of honey bee brood, causes chalkbrood disease in honey bee larvae worldwide. We used sister worker offspring of a single queen to minimize genetic. Differential expression of antioxidant system genes in honey. In general, the activity of antioxidant enzymes in a. Biomarkers of oxidative stress in atrazinetreated honey bees. The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, apis mellifera during two different seasons.
Apr, 2017 aupinel p, fortini d, michaud b, marolleau f, tasei jn, odoux jf 2007 toxicity of dimethoate and fenoxycarb to honey bee brood apis mellifera, using a new in vitro standardized feeding method. The objective of this study was to record the use and to evaluate the antioxidant, antihyperglycemic, and antidiabetic activity of apis mellifera bee tea, which is used by the guarani and kaiowa indigenous. Based on this, corona and robinson 2006 showed at least 38 genes coding for antioxidative proteins. Elevated expression of several traditional antioxidant encoding genes occurs. The honey bee expresses a cytoplasmic sod cuzn sod and a mitochondrial.
Nontarget inhibition of antioxidant enzymes in honey bees a. Antioxidative enzymes might help reduce this damage. However, the asian honey bee apis cerana is widely distributed throughout asia, and toxicity data are lacking for this species. Nacetylglucosaminidase, alkaline phosphatase, esterase, esterase lipase, leucine arylamidase, valine arylamidase, acid. Altmetric antioxidant enzymes in the honey bee,apis mellifera. The decline of honey bee apis mellifera colony numbers in recent years presents an economic and ecological threat to agriculture. Most studies that have investigated the effects of pesticides on honey bees have focused on the european honey bee apis mellifera hymenoptera. However, the physiological roles of several honey bee antioxidants have yet to be established. Insights into social insects from the genome of the. Objective several physical, biochemical and antioxidant properties of two nigerian stingless bee honey varieties melipona sp. Hsieh ys and hsu cy 20 oxidative stress and antioxidant enzyme activities in the trophocytes and fat cells of queen honey bees apis mellifera. Currently, populations of honey bees have declined in many regions of europe and north america vanengelsdorp and meixner, 2010. Insects free fulltext changes in antioxidant enzymes. In the honey bee, apis mellifera, mitochondrial activity in the larval fat body has been postulated as.
Dna comet lengths were 3 times longer in honey bees from insecticidetreatede national textile university, 38040 faisalabad, pa f department of agricultural development, democr g department of environmental sciences, comsats h i g h. Antioxidant enzymes in the honey bee, apis mellifera published in. In addition to producing honey, propolis and bee pollen, honey bees also produce bee venom. Sperm viability and gene expression in honey bee queens apis. The amount of pollen stored in honey bee, apis mellifera, colonies is a selectable trait. Tested enzymes showed variable affinity to substrates. Biological characteristics of the fungal pathogen and the molecular interactions between a. Honey bee antioxidant enzymes are of particular interest because of their potential involvement in some of the. Sperm storage and antioxidative enzyme expression in the honey bee, apis mellifera. Apr 10, 2020 the aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in apis mellifera and apis florea. Antioxidant, antihyperglycemic, and antidiabetic activity of.
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